Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells.
Methods Mol Biol
; 2199: 67-94, 2021.
Article
en En
| MEDLINE
| ID: mdl-33125645
ABSTRACT
This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing, and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 mL of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Baculoviridae
/
Vectores Genéticos
/
Proteínas de la Membrana
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido