Novel Type V-A CRISPR Effectors Are Active Nucleases with Expanded Targeting Capabilities.
CRISPR J
; 3(6): 454-461, 2020 12.
Article
en En
| MEDLINE
| ID: mdl-33146573
Cas12a enzymes are quickly being adopted for use in a variety of genome-editing applications. These programmable nucleases are part of adaptive microbial immune systems, the natural diversity of which has been largely unexplored. Here, we identified novel families of Type V-A CRISPR nucleases through a large-scale analysis of metagenomes collected from a variety of complex environments, and developed representatives of these systems into gene-editing platforms. The nucleases display extensive protein variation and can be programmed by a single-guide RNA with specific motifs. The majority of these enzymes are part of systems recovered from uncultivated organisms, some of which also encode a divergent Type V effector. Biochemical analysis uncovered unexpected protospacer adjacent motif diversity, indicating that these systems will facilitate a variety of genome-engineering applications. The simplicity of guide sequences and activity in human cell lines suggest utility in gene and cell therapies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Endodesoxirribonucleasas
/
Proteínas Asociadas a CRISPR
/
Edición Génica
Límite:
Humans
Idioma:
En
Revista:
CRISPR J
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos