The application of in-source fragmentation in ultra-high performance liquid chromatography-electrospray ionization -tandem mass spectrometry for pesticide residue analysis.
J Chromatogr A
; 1633: 461637, 2020 Dec 06.
Article
en En
| MEDLINE
| ID: mdl-33152553
ABSTRACT
In this study, in-source fragmentation in ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) was investigated and applied for pesticide residue analysis. Over 400 pesticides were tested, among which 26 pesticides were found to be sensitive to in-source fragmentation, producing 33 in-source fragments. The fragment pathways were studied and severe in-source fragmentation was observed due to the cleavage of C-O bond of carbamate, phosphate, ester, and ether groups, especially for isocarbophos and methoprene, leading to false negative results. High source temperatures could significantly increase the extent of in-source fragmentation. A multiple reaction monitoring (MRM) based multiresidue method was established for the pesticide residue analysis in vegetables and fruits, applying both pesticides and in-source fragments as precursors. The quantification ability of the method was validated and compared in terms of recovery, linearity, and the limit of quantification. In-source fragmentswere found to be more suitable than their parent pesticides as precursor ions for MRM analysis.
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Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Residuos de Plaguicidas
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Contaminación de Alimentos
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Cromatografía Líquida de Alta Presión
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Espectrometría de Masa por Ionización de Electrospray
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Espectrometría de Masas en Tándem
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Análisis de los Alimentos
Idioma:
En
Revista:
J Chromatogr A
Año:
2020
Tipo del documento:
Article