Protective effect of DLX6-AS1 silencing against cerebral ischemia/reperfusion induced impairments.
Aging (Albany NY)
; 12(22): 23096-23113, 2020 11 18.
Article
en En
| MEDLINE
| ID: mdl-33216728
ABSTRACT
In the present study, we investigated the role of lncRNA mus distal-less homeobox 6 antisense 1 (DLX6-AS1) during cerebral impairment induced by stroke. DLX6-AS1 levels were upregulated during ischemia/reperfusion (I/R) and downregulation of DLX6-AS1 reduced acute injury and ameliorated long-term neurological impairments induced by cerebral I/R in mice. Additionally, silencing of DLX6-AS1 significantly decreased the neuronal apoptosis in vivo and in vitro. Furthermore, inhibition of miRNA-149-3p led to enhance the apoptosis, which confirmed that DLX6-AS1 could sponge miR-149-3p. Finally, BOK was predicted to be the target of miR-149-3p using TargetScanVert software. And the silencing of DLX6-AS1 inhibited BOK expression both in vivo and in vitro, which was reversed by a miR-149-3p inhibitor. At meantime, BOK promoted OGD/R induced apoptosis in N2a cells. Therefore, this suggests that miR-149-3p sponging by DLX6-AS1 may lead to cerebral neuron I/R-induced impairments through upregulation of apoptotic BOK activity, which offers a new approach to the treatment of stroke impairment.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Conducta Animal
/
Daño por Reperfusión
/
Infarto de la Arteria Cerebral Media
/
Interferencia de ARN
/
ARN Largo no Codificante
/
Tratamiento con ARN de Interferencia
/
Neuronas
Límite:
Animals
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Humans
/
Male
Idioma:
En
Revista:
Aging (Albany NY)
Asunto de la revista:
GERIATRIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China