Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging.
J Vis Exp
; (166)2020 12 22.
Article
en En
| MEDLINE
| ID: mdl-33427237
ABSTRACT
Metabolomics, the study to identify and quantify small molecules and metabolites present in an experimental sample, has emerged as an important tool to investigate the biological activities during development and diseases. Metabolomics approaches are widely employed in the study of cancer, nutrition/diet, diabetes, and other physiological and pathological conditions involving metabolic processes. An advantageous tool that aids in metabolomic profiling advocated in this paper is matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Its ability to detect metabolites in situ without labeling, structural modifications, or other specialized reagents, such as those used in immunostaining, makes MALDI MSI a unique tool in advancing methodologies relevant in the field of metabolomics. An appropriate sample preparation process is critical to yield optimal results and will be the focus of this paper.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Métodos Analíticos de la Preparación de la Muestra
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Metabolómica
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Vis Exp
Año:
2020
Tipo del documento:
Article