Potassium bromate-induced cell model of age-related macular degeneration in vitro.
Mol Med Rep
; 23(3)2021 03.
Article
en En
| MEDLINE
| ID: mdl-33495823
ABSTRACT
Agerelated macular degeneration (AMD) progression occurs due to oxidative stress in retinal pigment epithelium (RPE) cells. To develop a new model of AMD, the present study investigated the effects of potassium bromate (KBrO3) on ARPE19 cells. Incubation with KBrO3 for 24 h significantly decreased ARPE19 cell viability in a concentrationdependent manner compared with the control group. The MTT and lactate dehydrogenase assay results indicated that KBrO3 induced cell apoptosis. Compared with the control group, KBrO3 treatment significantly decreased the Bcl2/Bax ratio, as determined via western blotting, and caspase3 mRNA expression levels. Fluorescence microscopy indicated the increased ROS levels in cells treated with KBrO3. Endogenous antioxidant enzyme activities, including superoxide dismutase and glutathione peroxidase, were significantly inhibited by KBrO3 compared with the control group. Moreover, the antioxidants tiron and phloroglucinol inhibited KBrO3mediated effects on ARPE19 cells in a dosedependent manner. Additionally, GPR109A is the binding site of 4hydroxynonenal (4HNE). KBrO3 displayed cytotoxic effects in 293 cells, which naturally lack the GPR109A gene, but these effects were not observed in 4HNEtreated 293 cells, suggesting that KBrO3 induced apoptosis without increasing endogenous 4HNE levels in cells. Moreover, the results suggested that KBrO3induced oxidative stress may activate STAT3 to increase VEGF expression in ARPE19 cells. Collectively, the results of the present study supported the potential use of KBrO3 to induce an in vitro model of AMD in ARPE19 cells.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bromatos
/
Apoptosis
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Degeneración Macular
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Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article