Diverse Eukaryotic CGG-Binding Proteins Produced by Independent Domestications of hAT Transposons.
Mol Biol Evol
; 38(5): 2070-2075, 2021 05 04.
Article
en En
| MEDLINE
| ID: mdl-33561217
The human transcription factor (TF) CGGBP1 (CGG-binding protein) is conserved only in amniotes and is believed to derive from the zf-BED and Hermes transposase DNA-binding domains (DBDs) of a hAT DNA transposon. Here, we show that sequence-specific DNA-binding proteins with this bipartite domain structure have resulted from dozens of independent hAT domestications in different eukaryotic lineages. CGGBPs display a wide range of sequence specificity, usually including preferences for CGG or CGC trinucleotides, whereas some bind AT-rich motifs. The CGGBPs are almost entirely nonsyntenic, and their protein sequences, DNA-binding motifs, and patterns of presence or absence in genomes are uncharacteristic of ancestry via speciation. At least eight CGGBPs in the coelacanth Latimeria chalumnae bind distinct motifs, and the expression of the corresponding genes varies considerably across tissues, suggesting tissue-restricted function.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
/
Proteínas de Unión al ADN
/
Peces
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Biol Evol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Canadá