Imaging-based screens of pool-synthesized cell libraries.
Nat Methods
; 18(4): 358-365, 2021 04.
Article
en En
| MEDLINE
| ID: mdl-33589838
ABSTRACT
Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Modelos Biológicos
Límite:
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos