Modeling Immunity with Rosetta: Methods for Antibody and Antigen Design.
Biochemistry
; 60(11): 825-846, 2021 03 23.
Article
en En
| MEDLINE
| ID: mdl-33705117
ABSTRACT
Structure-based antibody and antigen design has advanced greatly in recent years, due not only to the increasing availability of experimentally determined structures but also to improved computational methods for both prediction and design. Constant improvements in performance within the Rosetta software suite for biomolecular modeling have given rise to a greater breadth of structure prediction, including docking and design application cases for antibody and antigen modeling. Here, we present an overview of current protocols for antibody and antigen modeling using Rosetta and exemplify those by detailed tutorials originally developed for a Rosetta workshop at Vanderbilt University. These tutorials cover antibody structure prediction, docking, and design and antigen design strategies, including the addition of glycans in Rosetta. We expect that these materials will allow novice users to apply Rosetta in their own projects for modeling antibodies and antigens.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Modelos Biológicos
/
Anticuerpos
/
Antígenos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biochemistry
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos