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Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis.
Ozawa, Tatsuhiko; Kobayashi, Eiji; Hamana, Hiroshi; Nakamura, Tomoko; Lyu, Fulian; Hayashi, Atsushi; Muraguchi, Atsushi; Kishi, Hiroyuki.
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  • Ozawa T; Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
  • Kobayashi E; Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
  • Hamana H; Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
  • Nakamura T; Department of Ophthalmology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
  • Lyu F; Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences (Medicine), University of Toyama, Toyama, Japan.
  • Hayashi A; Department of Hematology, Collaborative Innovation Center of Hematology, The First Affiliated Hospital of Soochow University, Soochow University, Taipei, Taiwan, P. R. China.
  • Muraguchi A; Department of Ophthalmology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
  • Kishi H; Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
Eur J Immunol ; 51(7): 1850-1853, 2021 07.
Article en En | MEDLINE | ID: mdl-33728647
Generation of TCR-like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip-based Ab-secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single-cell level to obtain TCR-like antibodies; blocking procedure enables us to avoid the detection of non-TCR-like antibodies.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: Eur J Immunol Año: 2021 Tipo del documento: Article País de afiliación: Japón
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: Eur J Immunol Año: 2021 Tipo del documento: Article País de afiliación: Japón