A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis.
J Integr Plant Biol
; 63(8): 1462-1474, 2021 Aug.
Article
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| MEDLINE
| ID: mdl-33960113
ABSTRACT
In eukaryotes, histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation. HDA6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements (TEs) in Arabidopsis thaliana. HDA6 has been shown to participate in several complexes in plants, including a conserved SIN3 complex. Here, we uncover a novel protein complex containing HDA6, several Harbinger transposon-derived proteins (HHP1, SANT1, SANT2, SANT3, and SANT4), and MBD domain-containing proteins (MBD1, MBD2, and MBD4). We show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC, MAF4, and MAF5, resulting in a late flowering phenotype. Transcriptome deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes, TE silencing is unaffected in sant-null mutants. Our global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through deacetylation. Collectively, our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
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Histonas
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Genes de Plantas
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Arabidopsis
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Transposasas
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Proteínas de Arabidopsis
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Domesticación
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Histona Desacetilasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Integr Plant Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
China