Enhanced biodegradation of total petroleum hydrocarbons by implementing a novel two-step bioaugmentation strategy using indigenous bacterial consortium.

In the present study, a two-step bioaugmentation strategy (TSBS) was implemented by using indigenous bacterial consortium to enhance the degradation of total petroleum hydrocarbons (TPH) from petroleum refinery sludge (PRS). A bacterial consortium was developed using four indigenous isolated strains, Dietzia sp. IRB191, Dietzia sp. IRB192, Staphylococcus sp. BSM19 and Stenotrophomonas sp. IRB19 from PRS. The optimum conditions of pH, temperature, and sludge concentration were 7, 34 °C, and 2% (w/v), respectively, for maximum TPH degradation, obtained using one variable at a time approach. Under the optimal culture conditions, the developed consortium was inoculated twice to the culturing medium, at the beginning (0th day) and again on the 10th day for implementing a novel TSBS. The maximum TPH degradation of 91.5 ± 2.28% was found with TSBS, which was 1.18 times higher than that of SSBS (77.3 ± 2.6%) in 15 days of incubation. GC-FID study also confirmed that the TPH present in the PRS was effectively degraded by the bacterial consortium with TSBS. The TPH degradation by using TSBS proceeded according to the first-order kinetics with a rate constant of 0.155 d-1. Hence, biodegradation using a TSBS can be considered an effective and eco-friendly process for safe disposal of petroleum refinery sludge.