May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?
J Virol Methods
; 294: 114198, 2021 08.
Article
en En
| MEDLINE
| ID: mdl-34044003
ABSTRACT
OBJECTIVE:
This study aimed to investigate the prognostic value of viral load detected in the saliva of COVID-19 patients in the early stage of infection. STUDYDESIGN:
Oro-nasopharyngeal swab and saliva samples were collected from all patients simultaneously in the early stage of COVID-19. Viral loads were determined by extracting viral RNAs from saliva samples of patients whose ONP swabs were positive for SARS-CoV-2 by RT-qPCR. The demographic information, comorbidities, cycle threshold values, and one-month clinical courses were recorded and compared.RESULTS:
The patients' clinical course was evaluated for one month; 56 % of patients had mild disease, 26.4 % had moderate disease, 9.6 % had severe disease, and 8% had a critical/mortal disease. The average cycle threshold values of SARS-CoV-2 in saliva and ONP samples were measured as 22.28 and 24.19, respectively. Cycle threshold value of saliva was found to be significant in predicting disease severity (Eta coefficient 0.979). A statistically significant relationship was found between the disease's severity and the mean of ONP samples' Ct-values (p < 0.05). Gender, age, body mass index, and co-morbidities were compared with the severity of the disease; no statistically significant difference was found.CONCLUSION:
Viral load detected in saliva in the early period of COVID-19 infection may have a prognostic value in showing the disease's course in patients over 45-year-old. Saliva is an easily obtainable, reliable material for COVID-19 screening.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Saliva
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Carga Viral
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Prueba de Ácido Nucleico para COVID-19
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COVID-19
Tipo de estudio:
Prognostic_studies
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Screening_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Virol Methods
Año:
2021
Tipo del documento:
Article