Cardiac myosin-binding protein C interaction with actin is inhibited by compounds identified in a high-throughput fluorescence lifetime screen.

Bunch, Thomas A; Guhathakurta, Piyali; Lepak, Victoria C; Thompson, Andrew R; Kanassatega, Rhye-Samuel; Wilson, Anna; Thomas, David D; Colson, Brett A

Bunch, Thomas A; Guhathakurta, Piyali; Lepak, Victoria C; Thompson, Andrew R; Kanassatega, Rhye-Samuel; Wilson, Anna; Thomas, David D; Colson, Brett A.

Afiliación

Bunch TA; Department of Cellular & Molecular Medicine, University of Arizona, Tucson Arizona, USA.
Guhathakurta P; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Lepak VC; Department of Cellular & Molecular Medicine, University of Arizona, Tucson Arizona, USA.
Thompson AR; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Kanassatega RS; Department of Cellular & Molecular Medicine, University of Arizona, Tucson Arizona, USA.
Wilson A; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Thomas DD; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Colson BA; Department of Cellular & Molecular Medicine, University of Arizona, Tucson Arizona, USA. Electronic address: bcolson@arizona.edu.

J Biol Chem ; 297(1): 100840, 2021 07.

Article en En | MEDLINE | ID: mdl-34052227

Asunto(s)

Actinas/metabolismo; Proteínas Portadoras/metabolismo; Ensayos Analíticos de Alto Rendimiento; Miocardio/metabolismo; Actinas/química; Animales; Técnicas Biosensibles; Calorimetría; Fluorescencia; Transferencia Resonante de Energía de Fluorescencia; Humanos; Unión Proteica; Conejos; Sarcómeros/metabolismo; Factores de Tiempo

Palabras clave

actin; cardiac muscle; cardiac myosin-binding protein C (cMyBP-C); contractile proteins; fluorescence lifetime; high-throughput screening (HTS); library of pharmacologically active compounds (LOPAC); phosphorylation; protein kinase A (PKA); site-directed spectroscopy

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Portadoras / Actinas / Ensayos Analíticos de Alto Rendimiento / Miocardio Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google