High Pathological Reproducibility of Diet-induced Atherosclerosis in Microminipigs via Cloning Technology.
In Vivo
; 35(4): 2025-2033, 2021.
Article
en En
| MEDLINE
| ID: mdl-34182477
ABSTRACT
BACKGROUND/AIM:
The reproducibility of athero - sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring. MATERIALS ANDMETHODS:
Cloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups normal chow diet (NcD)-fed and high-fat/high-cholesterol diet (HcD)-fed groups. One of the F1-offsprings was subjected to cloning, and delivered males (F1-clones) were fed with HcD. After 8 weeks, all animals were necropsied for patho - physiological studies compared to non-cloned-microminipigs.RESULTS:
HcD-fed F1-offspring and F1-clones, but not NcD-fed F1-offspring, exhibited increased serum lipid levels and systemic atherosclerosis, which were comparable to those of HcD-fed non-cloned-microminipigs. Homogeneity of variance analysis demonstrated that standard deviation values of serum lipoprotein and aortic atherosclerosis area from HcD-fed animals decreased in F1-offspring and F1-clones.CONCLUSION:
HcD-induced atherogenesis was highly reproducible in F1-offsprings and F1-clones, indicating that the atherosclerosis-prone genomic background was preserved in the cloned-microminipigs, which can be used for studies on human atherosclerosis and related diseases.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Aterosclerosis
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
In Vivo
Asunto de la revista:
NEOPLASIAS
Año:
2021
Tipo del documento:
Article