Targeting the latent human cytomegalovirus reservoir for T-cell-mediated killing with virus-specific nanobodies.
Nat Commun
; 12(1): 4436, 2021 07 21.
Article
en En
| MEDLINE
| ID: mdl-34290252
ABSTRACT
Latent human cytomegalovirus (HCMV) infection is characterized by limited gene expression, making latent HCMV infections refractory to current treatments targeting viral replication. However, reactivation of latent HCMV in immunosuppressed solid organ and stem cell transplant patients often results in morbidity. Here, we report the killing of latently infected cells via a virus-specific nanobody (VUN100bv) that partially inhibits signaling of the viral receptor US28. VUN100bv reactivates immediate early gene expression in latently infected cells without inducing virus production. This allows recognition and killing of latently infected monocytes by autologous cytotoxic T lymphocytes from HCMV-seropositive individuals, which could serve as a therapy to reduce the HCMV latent reservoir of transplant patients.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Linfocitos T Citotóxicos
/
Latencia del Virus
/
Citomegalovirus
/
Anticuerpos de Dominio Único
Límite:
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Países Bajos