Characterization of a mouse model of chronic hydrocephalus induced by partial occlusion of the aqueduct of Sylvius in the adult brain.
J Neurosci Methods
; 362: 109294, 2021 10 01.
Article
en En
| MEDLINE
| ID: mdl-34293409
ABSTRACT
BACKGROUND:
Hydrocephalus is a neurologic disturbance produced by the abnormal production, circulation, and absorption of cerebrospinal fluid (CSF). Late-onset idiopathic aqueductal stenosis induces normal pressure hydrocephalus (NPH) in adults. To date, no animal model replicating chronic NPH is available to study the pathophysiological changes observed in these subjects. NEWMETHOD:
We performed and characterized a model that induces chronic hydrocephalus in the adult mouse brain by producing a pre-aqueductal semiobstruction using an acetate lamina inserted into the atrium of the aqueduct of Sylvius. After surgical procedure, we analyzed the hydrocephalus development on days 60 and 120 and sham-operated animals were used as controls. We included an additional group of hydrocephalus resolution in which we removed the obstruction and analyzed the morphological changes in the brain.RESULTS:
The hydrocephalus was fully established on day 60 after the obstruction and remained stable for 120 days. In all animals, the intracranial pressure remained ~4.08 mmHg and we did not find statistically significant differences between the hydrocephalus groups and controls. We did not find motor impairments and anxiety-like behaviors among groups and the analysis of microglia and astrogliosis revealed mild glial reactivity. COMPARISON WITH EXISTINGMETHODS:
This model generates a long-term ventricular enlargement with normal intracranial pressure and moderate glial reactivity. Importantly, this model allows the reversibility of ventricular enlargement after the removal of the obstructive film from the brain.CONCLUSIONS:
This mouse model may be useful to study the long-term cerebral alterations that occur during NPH or after its surgical resolution.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Acueducto del Mesencéfalo
/
Hidrocefalia
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2021
Tipo del documento:
Article
País de afiliación:
México