S-acylation of SARS-CoV-2 spike protein: Mechanistic dissection, in vitro reconstitution and role in viral infectivity.

Puthenveetil, Robbins; Lun, Cheng Man; Murphy, R Elliot; Healy, Liam B; Vilmen, Geraldine; Christenson, Eric T; Freed, Eric O; Banerjee, Anirban

S-acylation of SARS-CoV-2 spike protein: Mechanistic dissection, in vitro reconstitution and role in viral infectivity.

Puthenveetil, Robbins; Lun, Cheng Man; Murphy, R Elliot; Healy, Liam B; Vilmen, Geraldine; Christenson, Eric T; Freed, Eric O; Banerjee, Anirban.

Afiliación

Puthenveetil R; Section on Structural and Chemical Biology of Membrane Proteins, Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Lun CM; Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.
Murphy RE; Section on Structural and Chemical Biology of Membrane Proteins, Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Healy LB; Section on Structural and Chemical Biology of Membrane Proteins, Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Vilmen G; Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.
Christenson ET; Section on Structural and Chemical Biology of Membrane Proteins, Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Freed EO; Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.
Banerjee A; Section on Structural and Chemical Biology of Membrane Proteins, Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: anirban.banerjee@n

J Biol Chem ; 297(4): 101112, 2021 10.

Article en En | MEDLINE | ID: mdl-34428449

Asunto(s)

COVID-19/patología; SARS-CoV-2/metabolismo; Glicoproteína de la Espiga del Coronavirus/metabolismo; Acilación; Aciltransferasas/genética; Aciltransferasas/metabolismo; Secuencia de Aminoácidos; COVID-19/virología; Cisteína/metabolismo; Células HEK293; Humanos; Lipoilación; Mutagénesis Sitio-Dirigida; Proteínas Recombinantes/biosíntesis; Proteínas Recombinantes/química; Proteínas Recombinantes/aislamiento & purificación; SARS-CoV-2/aislamiento & purificación; Alineación de Secuencia; Glicoproteína de la Espiga del Coronavirus/química; Glicoproteína de la Espiga del Coronavirus/genética; Internalización del Virus

Palabras clave

SARS-CoV-2; in vitro reconstitution; infectious disease; membrane enzyme; membrane protein; posttranslational modification (PTM); protein palmitoylation; spike protein; viral protein; zDHHC enzyme

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glicoproteína de la Espiga del Coronavirus / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: J Biol Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

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