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Identification and Characterization of MIKCc-Type MADS-Box Genes in the Flower Organs of Adonis amurensis.
Ren, Lulu; Sun, Hongwei; Dai, Shengyue; Feng, Shuang; Qiao, Kun; Wang, Jingang; Gong, Shufang; Zhou, Aimin.
Afiliación
  • Ren L; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Sun H; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Dai S; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Feng S; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Qiao K; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Wang J; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Gong S; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
  • Zhou A; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci ; 22(17)2021 Aug 28.
Article en En | MEDLINE | ID: mdl-34502271
ABSTRACT
Adonis amurensis is a perennial herbaceous flower that blooms in early spring in northeast China, where the night temperature can drop to -15 °C. To understand flowering time regulation and floral organogenesis of A. amurensis, the MIKCc-type MADS (Mcm1/Agamous/ Deficiens/Srf)-box genes were identified and characterized from the transcriptomes of the flower organs. In this study, 43 non-redundant MADS-box genes (38 MIKCc, 3 MIKC*, and 2 Mα) were identified. Phylogenetic and conserved motif analysis divided the 38 MIKCc-type genes into three major classes ABCDE model (including AP1/FUL, AP3/PI, AG, STK, and SEPs/AGL6), suppressor of overexpression of constans1 (SOC1), and short vegetative phase (SVP). qPCR analysis showed that the ABCDE model genes were highly expressed mainly in flowers and differentially expressed in the different tissues of flower organs, suggesting that they may be involved in the flower organ identity of A. amurensis. Subcellular localization revealed that 17 full-length MADSs were mainly localized in the nucleus in Arabidopsis, the heterologous expression of three full-length SOC1-type genes caused early flowering and altered the expression of endogenous flowering time genes. Our analyses provide an overall insight into MIKCc genes in A. amurensis and their potential roles in floral organogenesis and flowering time regulation.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas de Plantas / Proteínas de Dominio MADS / Adonis / Flores Tipo de estudio: Diagnostic_studies Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas de Plantas / Proteínas de Dominio MADS / Adonis / Flores Tipo de estudio: Diagnostic_studies Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China