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Exogenous mitochondrial transfer and endogenous mitochondrial fission facilitate AML resistance to OxPhos inhibition.
Saito, Kaori; Zhang, Qi; Yang, Haeun; Yamatani, Kotoko; Ai, Tomohiko; Ruvolo, Vivian; Baran, Natalia; Cai, Tianyu; Ma, Helen; Jacamo, Rodrigo; Kuruvilla, Vinitha; Imoto, Junichi; Kinjo, Sonoko; Ikeo, Kazuho; Moriya, Kaori; Suzuki, Koya; Miida, Takashi; Kim, Yong-Mi; Vellano, Christopher P; Andreeff, Michael; Marszalek, Joseph R; Tabe, Yoko; Konopleva, Marina.
Afiliación
  • Saito K; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Zhang Q; Section of Leukemia Biology Research, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Yang H; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Yamatani K; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Ai T; Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.
  • Ruvolo V; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Baran N; Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Cai T; Section of Leukemia Biology Research, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Ma H; Section of Leukemia Biology Research, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Jacamo R; Section of Leukemia Biology Research, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Kuruvilla V; Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Imoto J; Section of Leukemia Biology Research, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Kinjo S; Center for Information Biology, National Institute of Genetics, Shizuoka, Japan.
  • Ikeo K; Center for Information Biology, National Institute of Genetics, Shizuoka, Japan.
  • Moriya K; Center for Information Biology, National Institute of Genetics, Shizuoka, Japan.
  • Suzuki K; Laboratory of Morphology and Image Analysis, and.
  • Miida T; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Kim YM; Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Vellano CP; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Andreeff M; Pediatrics and Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA.
  • Marszalek JR; TRACTION, Therapeutics Discovery Division, The University of Texas MD Anderson Cancer Center, Houston, TX; and.
  • Tabe Y; Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.
  • Konopleva M; TRACTION, Therapeutics Discovery Division, The University of Texas MD Anderson Cancer Center, Houston, TX; and.
Blood Adv ; 5(20): 4233-4255, 2021 10 26.
Article en En | MEDLINE | ID: mdl-34507353
Acute myeloid leukemia (AML) cells are highly dependent on oxidative phosphorylation (OxPhos) for survival, and they continually adapt to fluctuations in nutrient and oxygen availability in the bone marrow (BM) microenvironment. We investigated how the BM microenvironment affects the response to OxPhos inhibition in AML by using a novel complex I OxPhos inhibitor, IACS-010759. Cellular adhesion, growth, and apoptosis assays, along with measurements of expression of mitochondrial DNA and generation of mitochondrial reactive oxygen species indicated that direct interactions with BM stromal cells triggered compensatory activation of mitochondrial respiration and resistance to OxPhos inhibition in AML cells. Mechanistically, inhibition of OxPhos induced transfer of mitochondria derived from mesenchymal stem cells (MSCs) to AML cells via tunneling nanotubes under direct-contact coculture conditions. Inhibition of OxPhos also induced mitochondrial fission and increased functional mitochondria and mitophagy in AML cells. Mitochondrial fission is known to enhance cell migration, so we used electron microscopy to observe mitochondrial transport to the leading edge of protrusions of AML cells migrating toward MSCs. We further demonstrated that cytarabine, a commonly used antileukemia agent, increased mitochondrial transfer of MSCs to AML cells triggered by OxPhos inhibition. Our findings indicate an important role of exogenous mitochondrial trafficking from BM stromal cells to AML cells as well as endogenous mitochondrial fission and mitophagy in the compensatory adaptation of leukemia cells to energetic stress in the BM microenvironment.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fosforilación Oxidativa / Leucemia Mieloide Aguda Límite: Humans Idioma: En Revista: Blood Adv Año: 2021 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fosforilación Oxidativa / Leucemia Mieloide Aguda Límite: Humans Idioma: En Revista: Blood Adv Año: 2021 Tipo del documento: Article País de afiliación: Japón