CTCF knockout in zebrafish induces alterations in regulatory landscapes and developmental gene expression.
Nat Commun
; 12(1): 5415, 2021 09 13.
Article
en En
| MEDLINE
| ID: mdl-34518536
ABSTRACT
Coordinated chromatin interactions between enhancers and promoters are critical for gene regulation. The architectural protein CTCF mediates chromatin looping and is enriched at the boundaries of topologically associating domains (TADs), which are sub-megabase chromatin structures. In vitro CTCF depletion leads to a loss of TADs but has only limited effects over gene expression, challenging the concept that CTCF-mediated chromatin structures are a fundamental requirement for gene regulation. However, how CTCF and a perturbed chromatin structure impacts gene expression during development remains poorly understood. Here we link the loss of CTCF and gene regulation during patterning and organogenesis in a ctcf knockout zebrafish model. CTCF absence leads to loss of chromatin structure and affects the expression of thousands of genes, including many developmental regulators. Our results demonstrate the essential role of CTCF in providing the structural context for enhancer-promoter interactions, thus regulating developmental genes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas de Pez Cebra
/
Embrión no Mamífero
/
Técnicas de Inactivación de Genes
/
Factor de Unión a CCCTC
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
España