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Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing.
Sibbritt, Tennille; Schumann, Ulrike; Shafik, Andrew; Guarnacci, Marco; Clark, Susan J; Preiss, Thomas.
Afiliación
  • Sibbritt T; Department of Genome Sciences, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
  • Schumann U; Department of Genome Sciences, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
  • Shafik A; Department of Genome Sciences, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
  • Guarnacci M; Department of Genome Sciences, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
  • Clark SJ; Epigenetics Research Laboratory, Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.
  • Preiss T; St. Vincent Clinical School, University of New South Wales, Sydney, NSW, Australia.
Methods Mol Biol ; 2404: 375-392, 2022.
Article en En | MEDLINE | ID: mdl-34694621
ABSTRACT
Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m5C in DNA, but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification, and detection of candidate sites by sequencing on the Illumina MiSeq platform.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Análisis de Secuencia de ADN Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Análisis de Secuencia de ADN Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Australia