Influence of the expression vector and its elements on recombinant human prolactin synthesis in Escherichia coli; co-directional orientation of replication and transcription is highly critical.

Affonso, Regina; Suzuki, Miriam Fussae; Magalhães, Geraldo Santana; Bartolini, Paolo

Affonso, Regina; Suzuki, Miriam Fussae; Magalhães, Geraldo Santana; Bartolini, Paolo.

Afiliación

Affonso R; Biotechnology Center, Instituto de Pesquisas Energéticas e Nucleares, IPEN - CNEN/SP, São Paulo, SP, Brazil. Electronic address: reginaffonso@yahoo.com.br.
Suzuki MF; Biotechnology Center, Instituto de Pesquisas Energéticas e Nucleares, IPEN - CNEN/SP, São Paulo, SP, Brazil. Electronic address: mfsuzuki@ipen.br.
Magalhães GS; Immunopathology Laboratory, Butantan Institute, São Paulo, SP, Brazil. Electronic address: geraldo.magalhaes@butantan.gov.br.
Bartolini P; Biotechnology Center, Instituto de Pesquisas Energéticas e Nucleares, IPEN - CNEN/SP, São Paulo, SP, Brazil. Electronic address: pbartoli@ipen.br.

J Microbiol Methods ; 191: 106340, 2021 12.

Article en En | MEDLINE | ID: mdl-34715249

Asunto(s)

Escherichia coli/genética; Prolactina/biosíntesis; Prolactina/genética; Proteínas Recombinantes; Clonación Molecular; Escherichia coli/metabolismo; Regulación Bacteriana de la Expresión Génica; Humanos; Biosíntesis de Proteínas

Palabras clave

Co-directional collisions; Escherichia coli; Head-on collisions; Human prolactin; λP(L) and tac promoters

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Prolactina / Proteínas Recombinantes / Escherichia coli Límite: Humans Idioma: En Revista: J Microbiol Methods Año: 2021 Tipo del documento: Article

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