The use of biomarkers and HPV genotyping to improve diagnostic accuracy in women with a transformation zone type 3.
Br J Cancer
; 126(1): 91-99, 2022 01.
Article
en En
| MEDLINE
| ID: mdl-34716397
ABSTRACT
BACKGROUND:
Twenty percent of women referred to colposcopy have a type 3 transformation zone-where colposcopic assessment for high-grade dysplasia (CIN2+) is not possible. This study examines the effectiveness of HPV biomarkers and genotyping in combination with techniques that sample an endocervical TZ.METHODS:
A prospective diagnostic accuracy study. Women booked for large-loop excision (LLETZ) with squamous dyskaryosis, high-risk HPV and a TZ3 were recruited. Immediately prior to LLETZ samples were collected for p16/Ki-67 dual-stained cytology, HPV genotyping and H&E, p16- and Ki-67-stained endocervical curettings.RESULTS:
In women with low-grade screening (n = 64), 35.9% had CIN2+; dual-stained cytology had the greatest effect on the PPV of routine screening (76.1% vs 35.9%) and perfectly predicted the absence of CIN2+. In women with a high-grade screening result (n = 37); 75.6% had CIN2+ and dual-stained curettings improved the PPV (96.5 vs 75.6%).CONCLUSIONS:
With high-grade screening and a TZ3, LLETZ appears safest as three quarters have CIN2+ . Women with low-grade screening and a TZ3 have a twofold increased risk of CIN2+ when compared to women where the TZ is visible. The use of dual-stained cytology may help identify those women who can be safely offered surveillance and those who require treatment.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Papillomaviridae
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Biomarcadores de Tumor
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Displasia del Cuello del Útero
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Antígeno Ki-67
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Inhibidor p16 de la Quinasa Dependiente de Ciclina
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Infecciones por Papillomavirus
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Genotipo
Tipo de estudio:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Br J Cancer
Año:
2022
Tipo del documento:
Article
País de afiliación:
Reino Unido