HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.
Nucleic Acids Res
; 50(2): e10, 2022 01 25.
Article
en En
| MEDLINE
| ID: mdl-34734265
ABSTRACT
The interplay between three-dimensional chromosome organisation and genomic processes such as replication and transcription necessitates in vivo studies of chromosome dynamics. Fluorescent organic dyes are often used for chromosome labelling in vivo. The mode of binding of these dyes to DNA cause its distortion, elongation, and partial unwinding. The structural changes induce DNA damage and interfere with the binding dynamics of chromatin-associated proteins, consequently perturbing gene expression, genome replication, and cell cycle progression. We have developed a minimally-perturbing, genetically encoded fluorescent DNA label consisting of a (photo-switchable) fluorescent protein fused to the DNA-binding domain of H-NS - a bacterial nucleoid-associated protein. We show that this DNA label, abbreviated as HI-NESS (H-NS-based indicator for nucleic acid stainings), is minimally-perturbing to genomic processes and labels chromosomes in eukaryotic cells in culture, and in zebrafish embryos with preferential binding to AT-rich chromatin.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Proteínas Bacterianas
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Bioensayo
/
ADN Bacteriano
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Proteínas de Unión al ADN
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2022
Tipo del documento:
Article
País de afiliación:
Países Bajos