Evaluation of microfluidics-based droplet PCR combined with multiplex STR system in forensic science.
Electrophoresis
; 43(7-8): 848-856, 2022 04.
Article
en En
| MEDLINE
| ID: mdl-34842292
ABSTRACT
Because of its excellent monodispersity, high throughput, and low volume, microfluidics-based droplet PCR has become the core technology of digital PCR, next-generation sequencing, and other technology platforms. This study constructed a microfluidic water-in-oil droplet PCR system and amplified a commercially available forensic 22-plex short tandem repeat detection system. We analyzed the sensitivity, concordance, amplification efficiency of the droplet PCR, and influence factors of the above aspects. The droplet PCR showed high concordance with conventional bulk PCR and had high sensitivity as 0.125 ng. Furthermore, we observed the performance of droplet PCR in high-order mixed DNA. As the mixture ratios from 101 to 301, droplet PCR presented more mixture proportion (Mx) increased loci from 11 (57.89%) to 17 (89.47%). In the mixture ratios 201, 251, and 301, significant Mx differences between droplet PCR and bulk PCR were observed (p < 0.05). The results showed that the droplet PCR could improve the identification of the minor contributor's DNA in a two-person mixture and alleviate the imbalanced amplification problem. This study provides a reference and basis for the wide application of droplet PCR in forensic science.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Repeticiones de Microsatélite
/
Microfluídica
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Electrophoresis
Año:
2022
Tipo del documento:
Article