Prediction and validation of cross-protective candidate antigen of Hyalomma asiaticum cathepsin L between H. asiaticum and H. anatolicum.
Exp Appl Acarol
; 86(2): 283-298, 2022 Feb.
Article
en En
| MEDLINE
| ID: mdl-35133525
ABSTRACT
Hyalomma asiaticum and H. anatolicum are tick species in Eurasia and Africa with major medical and veterinary significance. Beside their direct pathogenic effects, H. asiaticum and H. anatolicum are vectors of important diseases of livestock and in some instances of zoonoses. In search of ways to address the increasing incidence of global acaricide resistance, tick control through vaccination is regarded as a sustainable alternative approach. Cathepsin L-like cysteine protease (CPL) is a potent hemoglobinase, and plays important roles in the digestion of blood acquired from a host. CPL from H. anatolicum (HanCPL) with high similarity (> 90%) for H. asiaticum CPL (HasCPL) were aligned by in silico analysis. After further in vitro validation, the anti-HasCPL sera have cross-reactivity between the different total native protein of life stages and tissues for H. asiaticum and H. anatolicum. Furthermore, we further confirmed that recombinant HasCPL (rHasCPL) immunized rabbits were partially cross-protected (54.8%) by H. anatolicum infestation.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Infestaciones por Garrapatas
/
Garrapatas
/
Ixodidae
/
Acaricidas
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Exp Appl Acarol
Asunto de la revista:
BIOLOGIA
/
PARASITOLOGIA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China