Orthotopic Transplantation of Mouse Mammary Epithelial Cells.
Methods Mol Biol
; 2471: 123-139, 2022.
Article
en En
| MEDLINE
| ID: mdl-35175594
ABSTRACT
The orthotopic transplantation assay has provided important insights into mammary development, stem cell function, and tumorigenesis. Technically, it consists in grafting mammary tissue fragments, organoids, mammospheres, or isolated cells into the fat pads of prepubertal mice from which the endogenous epithelium has been surgically removed, thereby allowing growth and differentiation of mammary epithelial cells in their physiological environment. Here, we describe how is conducted transplantation of epithelial fragments and cells isolated from mouse mammary glands, report the various approaches currently used to evaluate the regeneration and self-renewal properties of mammary stem cells, and highlight the strengths and limitations of this in vivo grafting assay.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Células Epiteliales
/
Glándulas Mamarias Animales
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2022
Tipo del documento:
Article
País de afiliación:
Francia