Canine MHC-class II antigens on B and T lymphocytes.
Tissue Antigens
; 27(2): 87-98, 1986 Feb.
Article
en En
| MEDLINE
| ID: mdl-3518148
ABSTRACT
MHC-class II antigens on canine lymphoid cells were detected with monoclonal antibodies (Mabs). On the basis of reactivity with resting or activated T lymphocytes two groups of Mabs were reported earlier. Non-activated T lymphocytes expressed MHC-class II antigens recognized by the first group whereas the second group recognized only MHC-class II antigens on activated T lymphocytes. In this study we analysed the reactivity pattern of both groups of Mabs with purified canine B lymphocytes. One- and two-colour immunoflowcytometric analysis, immunoprecipitation, immunohistology and MLC inhibition were performed. The Mabs which only reacted with activated T lymphocytes appeared to stain also purified sIg+ lymph node cells. Two-colour fluorescence and immunohistology confirmed the reactivity of these Mabs with B lymphocytes. Immunoprecipitation showed the bimolecular structure of these antigens. It was concluded that two subsets of MHC-class II antigens can be detected on canine lymphoid cells. One subset showed an aberrant distribution being expressed on both nonactivated and activated T and on B lymphocytes. The second subset of MHC-class II antigens could only be detected on B lymphocytes and activated T lymphocytes, a distribution pattern similar to that found in most other species, for instance man.
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Bases de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Linfocitos T
/
Perros
/
Antígenos de Histocompatibilidad
Límite:
Animals
Idioma:
En
Revista:
Tissue Antigens
Año:
1986
Tipo del documento:
Article