Expression and production of pigment epithelium-derived factor (PEDF) and PEDF receptor variants from mammalian and bacterial cells.

Bullock, Jeanee; Polato, Federica; Lulli, Daniel C; Sagar, Vatsala; Abaandou, Laura; Shiloach, Joseph; Becerra, S Patricia

Bullock, Jeanee; Polato, Federica; Lulli, Daniel C; Sagar, Vatsala; Abaandou, Laura; Shiloach, Joseph; Becerra, S Patricia.

Afiliación

Bullock J; Section of Protein Structure and Function-LRCMB, National Eye Institute, National Institutes of Health, Bethesda, MD, USA; Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, USA.
Polato F; Section of Protein Structure and Function-LRCMB, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Lulli DC; Section of Protein Structure and Function-LRCMB, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Sagar V; Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Abaandou L; Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA; Department of Chemistry and Biochemistry, George Mason University, Fairfax, VA, USA.
Shiloach J; Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Becerra SP; Section of Protein Structure and Function-LRCMB, National Eye Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: becerrap@nei.nih.gov.

Protein Expr Purif ; 194: 106072, 2022 06.

Article en En | MEDLINE | ID: mdl-35181508

Asunto(s)

Serpinas; Animales; Proteínas del Ojo/genética; Proteínas del Ojo/metabolismo; Humanos; Mamíferos; Factores de Crecimiento Nervioso/genética; Factores de Crecimiento Nervioso/metabolismo; Receptores de Neuropéptido; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Serpinas/genética; Serpinas/metabolismo

Palabras clave

Bacterial overexpression; Mammalian overexpression; PEDF; PNPLA2; Purification; Serpin

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Serpinas Límite: Animals / Humans Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

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