Viral Generation, Packaging, and Transduction on a Digital Microfluidic Platform.
Anal Chem
; 94(9): 4039-4047, 2022 03 08.
Article
en En
| MEDLINE
| ID: mdl-35192339
ABSTRACT
Viral-based systems are a popular delivery method for introducing exogenous genetic material into mammalian cells. Unfortunately, the preparation of lentiviruses containing the machinery to edit the cells is labor-intensive, with steps requiring optimization and sensitive handling. To mitigate these challenges, we introduce the first microfluidic method that integrates lentiviral generation, packaging, and transduction. The new method allows the production of viral titers between 106 and 107 (similar to macroscale production) and high transduction efficiency for hard-to-transfect cell lines. We extend the technique for gene editing applications and show how this technique can be used to knock out and knock down estrogen receptor geneâa gene prominently responsible for 70% of breast cancer cases. This new technique is automated with multiplexing capabilities, which have the potential to standardize the methods for viral-based genome engineering.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Revista:
Anal Chem
Año:
2022
Tipo del documento:
Article
País de afiliación:
Canadá