CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples.
Curr Protoc
; 2(2): e385, 2022 Feb.
Article
en En
| MEDLINE
| ID: mdl-35195954
ABSTRACT
The COVID-19 pandemic has taken a devastating human toll worldwide. The development of impactful guidelines and measures for controlling the COVID-19 pandemic requires continuous and widespread testing of suspected cases and their contacts through accurate, accessible, and reliable methods for SARS-CoV-2 detection. Here we describe a CRISPR-Cas13-based method for the detection of SARS-CoV-2. The assay is called CREST (Cas13-based, rugged, equitable, scalable testing), and is specific, sensitive, and highly accessible. As such, CREST may provide a low-cost and dependable alternative for SARS-CoV-2 surveillance. © 2022 Wiley Periodicals LLC. Basic Protocol Cas13-ased detection of SARS-CoV-2 genetic material using a real-time PCR detection system Alternate Protocol Cas13-based detection of SARS-CoV-2 genetic material using a fluorescence viewer Support Protocol 1 LwaCas13a purification Support Protocol 2 In vitro transcription of synthetic targets.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
SARS-CoV-2
/
COVID-19
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Curr Protoc
Año:
2022
Tipo del documento:
Article