Detection of ß-Lactoglobulin by a Porous Silicon Microcavity Biosensor Based on the Angle Spectrum.
Sensors (Basel)
; 22(5)2022 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-35271059
ABSTRACT
In this paper, carbon quantum dot-labelled ß-lactoglobulin antibodies were used for refractive index magnification, and ß-lactoglobulin was detected by angle spectroscopy. In this method, the detection light is provided by a He-Ne laser whose central wavelength is the same as that of the porous silicon microcavity device, and the light source was changed to a parallel beam to illuminate the porous silicon microcavity' surface by collimating beam expansion, and the reflected light was received on the porous silicon microcavity' surface by a detector. The angle corresponding to the smallest luminous intensity before and after the onset of immune response was measured by a detector for different concentrations of ß-lactoglobulin antigen and carbon quantum dot-labelled ß-lactoglobulin antibodies, and the relationship between the variation in angle before and after the immune response was obtained for different concentrations of the ß-lactoglobulin antigen. The results of the experiment present that the angle variations changed linearly with increasing ß-lactoglobulin antigen concentration before and after the immune response. The limit of detection of ß-lactoglobulin by this method was 0.73 µg/L, indicating that the method can be used to detect ß-lactoglobulin quickly and conveniently at low cost.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Silicio
/
Técnicas Biosensibles
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Año:
2022
Tipo del documento:
Article
País de afiliación:
China