Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13.
Stem Cell Res
; 61: 102757, 2022 05.
Article
en En
| MEDLINE
| ID: mdl-35339881
ABSTRACT
Angelman Syndrome is a rare neurodevelopmental disorder caused by several (epi)genetic alterations. The patients present strong neurological impairment due to the absence of a functional maternal UBE3A gene in neurons. Here, we generated and characterized a new induced pluripotent stem cell (iPSC) line from a female child with Angelman syndrome harbouring a class II deletion. iPSCs were reprogrammed from fibroblasts using Sendai viruses. The new iPSCs express pluripotency markers, are capable of trilineage in vitro differentiation and have the expected imprinting status of Angelman syndrome. These iPSCs are a valuable tool to elucidate the pathophysiological mechanisms associated with this disease.
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Bases de datos:
MEDLINE
Asunto principal:
Síndrome de Angelman
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Células Madre Pluripotentes Inducidas
Límite:
Child
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Female
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Humans
Idioma:
En
Revista:
Stem Cell Res
Año:
2022
Tipo del documento:
Article