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Rapid detection and discrimination of potentially toxigenic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis by multiplex real-time PCR and amplicon melting curve analysis.
Kawase, Jun; Sakai, Tomotake; Iwaki, Masaaki; Umeda, Kaoru; Fukuma, Aiko; Fujisawa, Naoki; Kawakami, Yuta; Hayashi, Hiroki; Wada, Mieko.
Afiliación
  • Kawase J; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan. Electronic address: kawase-jun@pref.shimane.lg.jp.
  • Sakai T; Shimane Prefectural Hamada Public Health Center, 254 kataniwa, Hamada City, Shimane 697-0041, Japan.
  • Iwaki M; Department of Bacteriology II, Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama City, Tokyo 208-0011, Japan.
  • Umeda K; Division of Microbiology, Osaka Institute of Public Health, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
  • Fukuma A; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.
  • Fujisawa N; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.
  • Kawakami Y; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.
  • Hayashi H; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.
  • Wada M; Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.
J Microbiol Methods ; 195: 106454, 2022 04.
Article en En | MEDLINE | ID: mdl-35351489
We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Corynebacterium pseudotuberculosis / Infecciones por Corynebacterium / Difteria Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Microbiol Methods Año: 2022 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Corynebacterium pseudotuberculosis / Infecciones por Corynebacterium / Difteria Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Microbiol Methods Año: 2022 Tipo del documento: Article