Quantification of tissue-specific protein translation in whole <i>C. elegans</i> using O-propargyl-puromycin labeling and fluorescence microscopy.

Somers, Hannah M; Fuqua, Jeremy H; Bonnet, Frédéric X A; Rollins, Jarod A

Quantification of tissue-specific protein translation in whole C. elegans using O-propargyl-puromycin labeling and fluorescence microscopy.

Somers, Hannah M; Fuqua, Jeremy H; Bonnet, Frédéric X A; Rollins, Jarod A.

Afiliación

Somers HM; Mount Desert Island Biological Laboratory, Davis Center for Regenerative Biology and Medicine, Salisbury Cove, ME 04609, USA.
Fuqua JH; Mount Desert Island Biological Laboratory, Davis Center for Regenerative Biology and Medicine, Salisbury Cove, ME 04609, USA.
Bonnet FXA; Mount Desert Island Biological Laboratory, Davis Center for Regenerative Biology and Medicine, Salisbury Cove, ME 04609, USA.
Rollins JA; Mount Desert Island Biological Laboratory, Davis Center for Regenerative Biology and Medicine, Salisbury Cove, ME 04609, USA.

Cell Rep Methods ; 2(4): 100203, 2022 04 25.

Article en En | MEDLINE | ID: mdl-35497499

Asunto(s)

Caenorhabditis elegans; Biosíntesis de Proteínas; Animales; Caenorhabditis elegans/genética; Puromicina/farmacología; Microscopía Fluorescente

Palabras clave

C. elegans; click chemistry; cuticle; elongation factors; fluorescence imaging; germline; initiation factors; longevity; puromycin; tissue specific; translation

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / Caenorhabditis elegans Límite: Animals Idioma: En Revista: Cell Rep Methods Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

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