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Rapid detection of the emerging tick-borne Tamdy virus by TaqMan-based real-time reverse transcription PCR.
Cui, Mingxue; Zhou, Hong; Zhang, Bin; Carr, Michael J; Guo, Moujian; Shi, Weifeng.
Afiliación
  • Cui M; Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China.
  • Zhou H; Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China.
  • Zhang B; College of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China.
  • Carr MJ; National Virus Reference Laboratory, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, N20 W10 Kita-ku, Sapporo 001-0020, Japan.
  • Guo M; Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China.
  • Shi W; Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China; School of Public Health, Shandong First Medical University & Shandong Academy of M
J Virol Methods ; 305: 114538, 2022 07.
Article en En | MEDLINE | ID: mdl-35513136
Tamdy virus (TAMV) is an emerging zoonotic tick-borne arbovirus in the genus Orthonairovirus. Reports of human infections with TAMV have been increasing and development of a rapid detection assay is thus urgently required. In the present study, singleplex and dual-target real-time reverse transcription PCR (qRT-PCR) assays were established for the detection of TAMV. Sensitivity and specificity were evaluated, which demonstrated high sensitivity for both the singleplex and dual-target qRT-PCR assays with no cross-reaction with common bunyaviruses and tick-borne viruses. The TaqMan-based qRT-PCR methodology established in this study can be employed for epidemiological surveillance and pathogenesis studies of TAMV.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Garrapatas / Orthobunyavirus / Thogotovirus Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: J Virol Methods Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Garrapatas / Orthobunyavirus / Thogotovirus Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: J Virol Methods Año: 2022 Tipo del documento: Article País de afiliación: China