Detecting cell-of-origin and cancer-specific methylation features of cell-free DNA from Nanopore sequencing.
Genome Biol
; 23(1): 158, 2022 07 15.
Article
en En
| MEDLINE
| ID: mdl-35841107
ABSTRACT
The Oxford Nanopore (ONT) platform provides portable and rapid genome sequencing, and its ability to natively profile DNA methylation without complex sample processing is attractive for point-of-care real-time sequencing. We recently demonstrated ONT shallow whole-genome sequencing to detect copy number alterations (CNAs) from the circulating tumor DNA (ctDNA) of cancer patients. Here, we show that cell type and cancer-specific methylation changes can also be detected, as well as cancer-associated fragmentation signatures. This feasibility study suggests that ONT shallow WGS could be a powerful tool for liquid biopsy.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ácidos Nucleicos Libres de Células
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ADN Tumoral Circulante
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Secuenciación de Nanoporos
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Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2022
Tipo del documento:
Article
País de afiliación:
Israel