Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy.
STAR Protoc
; 3(3): 101591, 2022 09 16.
Article
en En
| MEDLINE
| ID: mdl-35942346
ABSTRACT
Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nervios Periféricos
/
Técnicas Histológicas
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Japón