Increasing angiotensin-converting enzyme 1 regulated by histone 3 lysine 27 hyperacetylation in high-fat diet-induced hypertensive rat kidney.
J Hypertens
; 40(10): 1969-1978, 2022 10 01.
Article
en En
| MEDLINE
| ID: mdl-35969203
ABSTRACT
BACKGROUND:
Obesity is a key risk factor of hypertension. Angiotensin-converting enzyme 1 (ACE1) is a key enzyme involved in the renin-angiotensin-aldosterone system (RAAS), which contributes to obesity-related hypertension (OrHTN). Emerging evidence has shown that histone acetylation is also involved in OrHTN. As kidney is an effector organ that activates the RAAS by secreting renin after hypertension occurs, this study aimed to explore the regulatory role of histone acetylation on renal RAAS expression.METHODS:
Nineteen male Wistar rats were randomly divided into a control group ( n â=â9, fed normal chow) and a high-fat diet (HFD) group ( n â=â10, fed HFD for 16âweeks). The renal transcriptome and histone acetylation spectrum was analyzed by RNA sequencing and tandem mass spectrometry and was further confirmed by RT-qPCR, western blot, and immunohistochemistry. Then, chromatin immunoprecipitation (ChIP)-qPCR analysis was performed for the detection of DNA-protein interaction.RESULTS:
After 16-week HFD, the rats became obese with increased plasma triglyceride and high blood pressure. Increased ACE1 and histone 3 lysine 27 acetylation (H3K27ac) expression levels were found in OrHTN rat kidneys. The following ChIP-qPCR analysis illustrated that the upregulation of ACE1 transcription was mediated by increased H3K27ac.CONCLUSION:
H3K27ac could be an important histone acetylation site that activates renal ACE1 in HFD-induced hypertensive rats.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Dieta Alta en Grasa
/
Hipertensión
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
J Hypertens
Año:
2022
Tipo del documento:
Article