Using an on-site laboratory for fecal steroid analysis in wild white-faced capuchins.
Gen Comp Endocrinol
; 329: 114109, 2022 12 01.
Article
en En
| MEDLINE
| ID: mdl-36007549
ABSTRACT
Hormone laboratories located "on-site" where field studies are being conducted have a number of advantages. On-site laboratories allow hormone analyses to proceed in near-real-time, minimize logistics of sample permits/shipping, contribute to in-country capacity-building, and (our focus here) facilitate cross-site collaboration through shared methods and a shared laboratory. Here we provide proof-of-concept that an on-site hormone laboratory (the Taboga Field Laboratory, located in the Taboga Forest Reserve, Costa Rica) can successfully run endocrine analyses in a remote location. Using fecal samples from wild white-faced capuchins (Cebus imitator) from three Costa Rican forests, we validate the extraction and analysis of four steroid hormones (glucocorticoids, testosterone, estradiol, progesterone) across six assays (DetectX® and ISWE, all from Arbor Assays). Additionally, as the first collaboration across three long-term, wild capuchin field sites (Lomas Barbudal, Santa Rosa, Taboga) involving local Costa Rican collaborators, this laboratory can serve as a future hub for collaborative exchange.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Cebus capucinus
Límite:
Animals
País/Región como asunto:
America central
/
Costa rica
Idioma:
En
Revista:
Gen Comp Endocrinol
Año:
2022
Tipo del documento:
Article