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Integrated and automated high-throughput purification of libraries on microscale.
Ginsburg-Moraff, Carol; Grob, Jonathan; Chin, Karl; Eastman, Grant; Wildhaber, Sandra; Bayliss, Mark; Mues, Heinrich M; Palmieri, Marco; Poirier, Jennifer; Reck, Marcel; Luneau, Alexandre; Rodde, Stephane; Reilly, John; Wagner, Trixie; Brocklehurst, Cara E; Wyler, René; Dunstan, David; Marziale, Alexander N.
Afiliación
  • Ginsburg-Moraff C; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA. Electronic address: carol.ginsburg-moraff@novartis.com.
  • Grob J; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
  • Chin K; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
  • Eastman G; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
  • Wildhaber S; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Bayliss M; Virscidian Inc., Cary, NC 27511, USA.
  • Mues HM; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Palmieri M; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Poirier J; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA.
  • Reck M; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Luneau A; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Rodde S; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Reilly J; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Wagner T; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Brocklehurst CE; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Wyler R; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland.
  • Dunstan D; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Cambridge, MA 02139, USA. Electronic address: david.dunstan@novartis.com.
  • Marziale AN; Global Discovery Chemistry, Novartis Institutes for Biomedical Research, Novartis Pharma AG., Fabrikstrasse 1, Basel 4056, Switzerland. Electronic address: alexander.marziale@gmx.de.
SLAS Technol ; 27(6): 350-360, 2022 Dec.
Article en En | MEDLINE | ID: mdl-36028206
ABSTRACT
We herein report the development of an automation platform for rapid purification and quantification of chemical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on analytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bibliotecas de Moléculas Pequeñas Idioma: En Revista: SLAS Technol Año: 2022 Tipo del documento: Article
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bibliotecas de Moléculas Pequeñas Idioma: En Revista: SLAS Technol Año: 2022 Tipo del documento: Article