Dynamic Spatial-temporal Expression Ratio of X Chromosome to Autosomes but Stable Dosage Compensation in Mammals.
Genomics Proteomics Bioinformatics
; 2022 Aug 27.
Article
en En
| MEDLINE
| ID: mdl-36031057
ABSTRACT
In the evolutionary model of dosage compensation, per-allele expression level of the X chromosome has been proposed to have twofold up-regulation to compensate its dose reduction in males (XY) compared to females (XX). However, the expression regulation of X-linked genes is still controversial, and comprehensive evaluations are still lacking. By integrating multi-omics datasets in mammals, we investigated the expression ratios including X to autosomes (XAA ratio) and X to orthologs (XXX ratio) at the transcriptome, translatome, and proteome levels. We revealed a dynamic spatial-temporal XAA ratio during development in humans and mice. Meanwhile, by tracing the evolution of orthologous gene expression in chickens, platypuses, and opossums, we found a stable expression ratio of X-linked genes in humans to their autosomal orthologs in other species (XXX ≈ 1) across tissues and developmental stages, demonstrating stable dosage compensation in mammals. We also found that different epigenetic regulations contributed to the high tissue specificity and stage specificity of X-linked gene expression, thus affecting XAA ratios. It could be concluded that the dynamics of XAA ratios were attributed to the different gene contents and expression preferences of the X chromosome, rather than the stable dosage compensation.
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Bases de datos:
MEDLINE
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En
Revista:
Genomics Proteomics Bioinformatics
Asunto de la revista:
BIOQUIMICA
/
GENETICA
/
INFORMATICA MEDICA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China