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Antibiotics targeting bacterial protein synthesis reduce the lytic activity of bacteriophages.
Vashisth, Medhavi; Yashveer, Shikha; Anand, Taruna; Virmani, Nitin; Bera, Bidhan Chand; Vaid, Rajesh Kumar.
Afiliación
  • Vashisth M; Bacteriophage Laboratory, National Centre for Veterinary Type Cultures, ICAR - National Research Centre on Equines, Sirsa Road, Hisar, Haryana 125001, India; Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 1250
  • Yashveer S; Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004, India.
  • Anand T; Bacteriophage Laboratory, National Centre for Veterinary Type Cultures, ICAR - National Research Centre on Equines, Sirsa Road, Hisar, Haryana 125001, India. Electronic address: Taruna.Anand@icar.gov.in.
  • Virmani N; Bacteriophage Laboratory, National Centre for Veterinary Type Cultures, ICAR - National Research Centre on Equines, Sirsa Road, Hisar, Haryana 125001, India.
  • Bera BC; Bacteriophage Laboratory, National Centre for Veterinary Type Cultures, ICAR - National Research Centre on Equines, Sirsa Road, Hisar, Haryana 125001, India.
  • Vaid RK; Bacteriophage Laboratory, National Centre for Veterinary Type Cultures, ICAR - National Research Centre on Equines, Sirsa Road, Hisar, Haryana 125001, India.
Virus Res ; 321: 198909, 2022 11.
Article en En | MEDLINE | ID: mdl-36057417
Combination therapy of bacteriophages and antibiotics requires careful selection of specific antibiotics as it is crucial towards determining the success of phage therapy to treat multiple drug-resistant bacterial infections. So, we examined how different antibiotics can affect phage lytic activity when used in combination against targeted bacteria. Various antibiotics targeting bacterial protein synthesis pathways were tested for their bactericidal action in combination with bacteriophages of Acinetobacter baumannii (φAB145, φAB182), Staphylococcus aureus (φSA115, φSA116) and Salmonella Typhimurium (φST143, φST188). The phages displayed highly significant antagonism with most of the protein/ribosomal machinery targeting antibiotics: φSA115 (13/13); φSA116 (13/13); φST143 (11/13); φAB145 (11/13); φST188 (9/13); φAB182 (7/13). To validate this antagonistic effect, synergy assessment of these phages with gentamicin (GEN) and tetracycline (TE) was performed using time kill curve assays and counting the remaining viable bacterial cells at the end of the experiment. An increase in bacterial turbidity in phage-antibiotic combination groups was observed as compared to the treatment with phages individually. Also, GEN exhibited 4.22, 5.90, 2.02, 3.15, 2.68, and 2.60 log proliferation in viable cell count, respectively, for φSA115, φSA116, φST145, φAB182, φST143 and φAB188 in combination group in comparison to their individual actions. TE supplementation also led to 2.40, 4.90, 1.61, 2.73, 3.93, and 1.81 log increments in viable bacterial count when combined with φSA115, φSA116, φST145, φAB182, φST143 and φAB188, respectively. This study concludes that antibiotics targeting the bacterial protein biosynthetic machinery may lead to a reduction in the lytic activity of bacteriophages, thus lowering their therapeutic potential. Hence, such compounds must be carefully screened before their employment in combination treatment regimens.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bacteriófagos Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2022 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bacteriófagos Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2022 Tipo del documento: Article