Histomorphometric analysis of the endometrium in an ectopic model of endometriosis in mice.
Gynecol Endocrinol
; 38(10): 874-878, 2022 Oct.
Article
en En
| MEDLINE
| ID: mdl-36067792
ABSTRACT
Objective:
Evaluate histomorphometry of ectopic and eutopic endometrial tissues in receptor mice.Method:
Eighteen female Balb/c were divided into 3 groups, 6 animals each GI Control, no procedure; GII - Sham, animals that had the same procedures as GIII without receiving the ectopic endometrial implant. Instead, they received saline solution; GIII - endometriosis model, animals had surgical intervention with an ectopic endometrial implant. GI and GIII mice were treated with 17ß-estradiol, 100 µg/kg each. All animals were euthanized to collect uterine horns, which were fixed in 4% paraformaldehyde, embedded in paraffin, stained with Hematoxilin and Eosin and submitted to histomorphometric analyzes. Data underwent one-way ANOVA followed by Tukey's test.Results:
Local tissue growth, showing important lesions and adhesions, as well as dark cysts were noticed. In GIII group, there was an increase in number of blood vessels and glands (GIII ≥ GI and GIII p > .001). Thickening of the GIII endometrial epithelial was also evident (GIII ≥ GI and GIII. p > .001). We also noticed an increase in the number of eosinophils (GIII (GIII ≥ GI and GIII. p > .001).Conclusion:
Easy to perform model, capable of reproducing morphological endometriosis characteristics. From our findings, there was an increase of endometrial thickness as well as an increase in the eosinophils population.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Endometriosis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Gynecol Endocrinol
Asunto de la revista:
ENDOCRINOLOGIA
/
GINECOLOGIA
Año:
2022
Tipo del documento:
Article
País de afiliación:
Brasil