Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model.
STAR Protoc
; 3(4): 101816, 2022 12 16.
Article
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| MEDLINE
| ID: mdl-36386888
ABSTRACT
Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPEnC) and epithelial papilloma (HIBCPP) cells grown on opposite sides of filter supports. In this protocol, we describe the preparation of this model, the measurement of transepithelial electrical resistance (TEER), and immunofluorescence imaging-based analysis to determine the barrier function. For complete details on the use and execution of this protocol, please refer to Muranyi et al. (2022).
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Plexo Coroideo
/
Células Endoteliales
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Alemania