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Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model.
Herold, Rosanna; Borkowski, Julia; Schwerk, Christian; Stump-Guthier, Carolin; Lampe, Marko; Ishikawa, Hiroshi; Muranyi, Walter; Schroten, Horst.
Afiliación
  • Herold R; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Borkowski J; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Schwerk C; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Stump-Guthier C; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Lampe M; European Molecular Biology Laboratory, Advanced Light Microscopy Core Facility, 69117 Heidelberg, Germany.
  • Ishikawa H; Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
  • Muranyi W; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany. Electronic address: walter.muranyi@medma.uni-heidelberg.de.
  • Schroten H; Pediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany; European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
STAR Protoc ; 3(4): 101816, 2022 12 16.
Article en En | MEDLINE | ID: mdl-36386888
ABSTRACT
Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPEnC) and epithelial papilloma (HIBCPP) cells grown on opposite sides of filter supports. In this protocol, we describe the preparation of this model, the measurement of transepithelial electrical resistance (TEER), and immunofluorescence imaging-based analysis to determine the barrier function. For complete details on the use and execution of this protocol, please refer to Muranyi et al. (2022).
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Plexo Coroideo / Células Endoteliales Límite: Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article País de afiliación: Alemania
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Plexo Coroideo / Células Endoteliales Límite: Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article País de afiliación: Alemania