Fast and Multiplexed Super Resolution Imaging of Fixed and Immunostained Cells with DNA-PAINT-ERS.
Curr Protoc
; 2(11): e618, 2022 Nov.
Article
en En
| MEDLINE
| ID: mdl-36426921
ABSTRACT
Recent advances in super resolution microscopy have enabled imaging at the 10-20 nm scale on a light microscope, providing unprecedented details of native biological structures and processes in intact and hydrated samples. Of the existing strategies, DNA points accumulation in imaging nanoscale topography (DNA-PAINT) affords convenient multiplexing, an important feature in interrogating complex biological systems. A practical limitation of DNA-PAINT, however, has been the slow imaging speed. In its original form, DNA-PAINT imaging of each target takes tens of minutes to hours to complete. To address this challenge, several improved implementations have been introduced. These include DNA-PAINT-ERS (where E = ethylene carbonate; R = repeat sequence; S = spacer), a set of strategies that leads to both accelerated DNA-PAINT imaging speed and improved image quality. With DNA-PAINT-ERS, imaging of typical cellular targets such as microtubules takes only 5-10 min. Importantly, DNA-PAINT-ERS also facilitates multiplexing and can be easily integrated into current workflows for fluorescence staining of biological samples. Here, we provide a detailed, step-by-step guide for fast and multiplexed DNA-PAINT-ERS imaging of fixed and immunostained cells grown on glass substrates as adherent monolayers. The protocol should be readily extended to biological samples of a different format (for example tissue sections) or staining mechanisms (for example using nanobodies). © 2022 Wiley Periodicals LLC. Basic Protocol 1 Preparation of probes for DNA-PAINT-ERS Basic Protocol 2 Sample preparation for imaging membrane targets with DNA-PAINT-ERS in fixed cells Alternate Protocol Immunostaining of extracted U2OS cells Basic Protocol 3 Super resolution image acquisition and analysis.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN
/
Microtúbulos
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Curr Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos