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GTP-stimulated membrane fission by the N-BAR protein AMPH-1.
Kustigian, Lauren; Gong, Xue; Gai, Wei; Thongchol, Jirapat; Zhang, Junjie; Puchalla, Jason; Carr, Chavela M; Rye, Hays S.
Afiliación
  • Kustigian L; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Gong X; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Gai W; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Thongchol J; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Zhang J; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Puchalla J; Department of Physics, Princeton University, Princeton, New Jersey, USA.
  • Carr CM; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
  • Rye HS; Department of Biochemistry and Biophysics, Texas A&M University, Texas, USA.
Traffic ; 24(1): 34-47, 2023 01.
Article en En | MEDLINE | ID: mdl-36435193
ABSTRACT
Membrane-enclosed transport carriers sort biological molecules between stations in the cell in a dynamic process that is fundamental to the physiology of eukaryotic organisms. While much is known about the formation and release of carriers from specific intracellular membranes, the mechanism of carrier formation from the recycling endosome, a compartment central to cellular signaling, remains to be resolved. In Caenorhabditis elegans, formation of transport carriers from the recycling endosome requires the dynamin-like, Eps15-homology domain (EHD) protein, RME-1, functioning with the Bin/Amphiphysin/Rvs (N-BAR) domain protein, AMPH-1. Here we show, using a free-solution single-particle technique known as burst analysis spectroscopy (BAS), that AMPH-1 alone creates small, tubular-vesicular products from large, unilamellar vesicles by membrane fission. Membrane fission requires the amphipathic H0 helix of AMPH-1 and is slowed in the presence of RME-1. Unexpectedly, AMPH-1-induced membrane fission is stimulated in the presence of GTP. Furthermore, the GTP-stimulated membrane fission activity seen for AMPH-1 is recapitulated by the heterodimeric N-BAR amphiphysin protein from yeast, Rvs161/167p, strongly suggesting that GTP-stimulated membrane fission is a general property of this important class of N-BAR proteins.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Endosomas / Endocitosis Límite: Animals Idioma: En Revista: Traffic Asunto de la revista: FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Endosomas / Endocitosis Límite: Animals Idioma: En Revista: Traffic Asunto de la revista: FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos