Cellular and Molecular Mechanisms of MEK1 Inhibitor-Induced Cardiotoxicity.
JACC CardioOncol
; 4(4): 535-548, 2022 Nov.
Article
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| MEDLINE
| ID: mdl-36444237
ABSTRACT
Background:
Trametinib is a MEK1 (mitogen-activated extracellular signal-related kinase kinase 1) inhibitor used in the treatment of BRAF (rapid accelerated fibrosarcoma B-type)-mutated metastatic melanoma. Roughly 11% of patients develop cardiomyopathy following long-term trametinib exposure. Although described clinically, the molecular landscape of trametinib cardiotoxicity has not been characterized.Objectives:
The aim of this study was to test the hypothesis that trametinib promotes widespread transcriptomic and cellular changes consistent with oxidative stress and impairs cardiac function.Methods:
Mice were treated with trametinib (1 mg/kg/d). Echocardiography was performed pre- and post-treatment. Gross, histopathologic, and biochemical assessments were performed to probe for molecular and cellular changes. Human cardiac organoids were used as an in vitro measurement of cardiotoxicity and recovery.Results:
Long-term administration of trametinib was associated with significant reductions in survival and left ventricular ejection fraction. Histologic analyses of the heart revealed myocardial vacuolization and calcification in 28% of animals. Bulk RNA sequencing identified 435 differentially expressed genes and 116 differential signaling pathways following trametinib treatment. Upstream gene analysis predicted interleukin-6 as a regulator of 17 relevant differentially expressed genes, suggestive of PI3K/AKT and JAK/STAT activation, which was subsequently validated. Trametinib hearts displayed elevated markers of oxidative stress, myofibrillar degeneration, an 11-fold down-regulation of the apelin receptor, and connexin-43 mislocalization. To confirm the direct cardiotoxic effects of trametinib, human cardiac organoids were treated for 6 days, followed by a 6-day media-only recovery. Trametinib-treated organoids exhibited reductions in diameter and contractility, followed by partial recovery with removal of treatment.Conclusions:
These data describe pathologic changes observed in trametinib cardiotoxicity, supporting the exploration of drug holidays and alternative pharmacologic strategies for disease prevention.
APJ, apelin receptor; BRAF, rapid accelerated fibrosarcoma B-type; CX43, connexin-43; ECM, extracellular matrix; ERK1/2, extracellular signal-regulated protein kinase 1/2; IL, interleukin; MEK1; MEK1, mitogen-activated extracellular signal-related kinase kinase 1; cardiomyopathy; cardiotoxicity; hCO, human cardiac organoid; hERG, human ether-ago-go; trametinib
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Bases de datos:
MEDLINE
Idioma:
En
Revista:
JACC CardioOncol
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos