Facilitating viral vector movement enhances heterologous protein production in an established plant system.
Plant Biotechnol J
; 21(3): 635-645, 2023 03.
Article
en En
| MEDLINE
| ID: mdl-36511837
ABSTRACT
Molecular farming technology using transiently transformed Nicotiana plants offers an economical approach to the pharmaceutical industry to produce an array of protein targets including vaccine antigens and therapeutics. It can serve as a desirable alternative approach for those proteins that are challenging or too costly to produce in large quantities using other heterologous protein expression systems. However, since cost metrics are such a critical factor in selecting a production host, any system-wide modifications that can increase recombinant protein yields are key to further improving the platform and making it applicable for a wider range of target molecules. Here, we report on the development of a new approach to improve target accumulation in an established plant-based expression system that utilizes viral-based vectors to mediate transient expression in Nicotiana benthamiana. We show that by engineering the host plant to support viral vectors to spread more effectively between host cells through plasmodesmata, protein target accumulation can be increased by up to approximately 60%.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus del Mosaico del Tabaco
Idioma:
En
Revista:
Plant Biotechnol J
Asunto de la revista:
BIOTECNOLOGIA
/
BOTANICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos